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Sarstedt cm2 culture flasks
Cm2 Culture Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cm2+culture+flasks/flasks+t75/pm42274582-46-19-22
Average 86 stars, based on 1 article reviews
cm2 culture flasks - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Cell Culture:

Article Title: Inhibition of Epithelial-Mesenchymal Transition and Metastasis by Combined TGFbeta Knockdown and Metformin Treatment in a Canine Mammary Cancer Xenograft Model.
Article Snippet: Antibodies used in this study were as follows: vimentin (#M0725) from DAKO (Carpinteria, CA, USA); N-cadherin (#sc7939) from Santa Cruz Biotechnology (Dallas, Texas, USA), E-cadherin (#3195S) from Cell Signaling Technology (Danvers, Massachusetts, USA), claudin-7 (ab27487) from Abcam (Cambridge, UK) and Alexa-Fluor®-conjugated secondary antibodies from Life Technologies (Eugene, OR, USA). .. The metastatic canine mammary tumor cell line CF41 (ATCC, Manassas, VA, USA) was cultured in 75 cm2 culture flasks (Sarstedt, Nümbrecht, Germany) with Dulbecco’s modified Eagle’s medium – high glucose (DMEM) (Cultilab, Campinas, SP, Brazil) supplemented with 10% fetal bovine serum (FBS) (Cultilab, Campinas, SP, Brazil), penicillin (100 IU/ mL) and streptomycin (10 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) in a humidified incubator at 5% CO2 and 37 °C. ..

Article Title: Inhibition of Epithelial-mesenchymal Transition in Response to Treatment with Metformin and Y27632 in Breast Cancer Cell Lines.
Article Snippet: Background: ROCK-1 expression is associated with the malignant character of tumors, while inhibiting this molecule results in a significant suppression of tumor metastasis.. Likewise, transforming growth factor beta (TGF-β) is associated with this malignancy by having the ability to induce epithelial-mesenchymal transition (EMT).. Metformin, a drug used in the treatment of diabetes, has previously been shown to inhibit EMT in breast cancer cells.

Article Title: The influence of soluble epoxide hydrolase inhibition and their PUFA-derived epoxides in osteoblast bone metabolism: an in vitro study.
Article Snippet: EpFAs are crucial mediators in resolving inflammation and regulating various biological processes.. However, their activity is constrained by the rapid metabolism mediated by the soluble epoxide hydrolase (sEH), which converts EpFAs into inactive or even pro-inflammatory diols.. Nevertheless, the specific effects of soluble epoxide hydrolase inhibition (sEHI) and EpFAs on osteogenic cell metabolism remain unclear.

Modification:

Article Title: Inhibition of Epithelial-Mesenchymal Transition and Metastasis by Combined TGFbeta Knockdown and Metformin Treatment in a Canine Mammary Cancer Xenograft Model.
Article Snippet: Antibodies used in this study were as follows: vimentin (#M0725) from DAKO (Carpinteria, CA, USA); N-cadherin (#sc7939) from Santa Cruz Biotechnology (Dallas, Texas, USA), E-cadherin (#3195S) from Cell Signaling Technology (Danvers, Massachusetts, USA), claudin-7 (ab27487) from Abcam (Cambridge, UK) and Alexa-Fluor®-conjugated secondary antibodies from Life Technologies (Eugene, OR, USA). .. The metastatic canine mammary tumor cell line CF41 (ATCC, Manassas, VA, USA) was cultured in 75 cm2 culture flasks (Sarstedt, Nümbrecht, Germany) with Dulbecco’s modified Eagle’s medium – high glucose (DMEM) (Cultilab, Campinas, SP, Brazil) supplemented with 10% fetal bovine serum (FBS) (Cultilab, Campinas, SP, Brazil), penicillin (100 IU/ mL) and streptomycin (10 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) in a humidified incubator at 5% CO2 and 37 °C. ..

Article Title: Inhibition of Epithelial-mesenchymal Transition in Response to Treatment with Metformin and Y27632 in Breast Cancer Cell Lines.
Article Snippet: Background: ROCK-1 expression is associated with the malignant character of tumors, while inhibiting this molecule results in a significant suppression of tumor metastasis.. Likewise, transforming growth factor beta (TGF-β) is associated with this malignancy by having the ability to induce epithelial-mesenchymal transition (EMT).. Metformin, a drug used in the treatment of diabetes, has previously been shown to inhibit EMT in breast cancer cells.

Incubation:

Article Title: Scalable synovial fibroblast sources enable reproducible basal synovial organoid formation: an in vitro platform study.
Article Snippet: .. SFMCs were incubated for 48 h in 25 cm2 culture flasks (Sarstedt, Denmark) containing DMEM media (Sigma-Aldrich, Germany) supplemented with 10% heat-inactivated fetal calf serum (Sigma-Aldrich, Germany) and 1% penicillin/ streptomycin/ glutamine (Gibco, USA). .. Cells in suspension were then removed with 1 × PBS wash. Adherent cells were further incubated in supplemented DMEM media (changed every 3–4 days) until > 70% confluent and thus ready for passaging with a trypsin (Gibco, USA)-EDTA (Invitrogen, USA) detachment buffer.

Multiple Displacement Amplification:

Article Title: Inhibition of Epithelial-mesenchymal Transition in Response to Treatment with Metformin and Y27632 in Breast Cancer Cell Lines.
Article Snippet: Background: ROCK-1 expression is associated with the malignant character of tumors, while inhibiting this molecule results in a significant suppression of tumor metastasis.. Likewise, transforming growth factor beta (TGF-β) is associated with this malignancy by having the ability to induce epithelial-mesenchymal transition (EMT).. Metformin, a drug used in the treatment of diabetes, has previously been shown to inhibit EMT in breast cancer cells.



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